Prep HPLC Column, RP C4, Wide Pore, 10um, 300A. 21.2mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C4 Butyl Wide Pore is a reversed-phase HPLC stationary phase manufactured using high-purity silica with traditional butyl (C4) ligand bonding and complete double endcapping. The 300 Å pore structure is specifically engineered to improve accessibility for proteins, peptides, enzymes, and other large biomolecules, enabling efficient mass transfer and excellent recovery during purification. The moderate hydrophobicity of the C4 ligand provides effective retention while helping maintain biomolecular integrity throughout preparative workflows.
68004-P21-50-3M is a preparative HPLC column with dimensions of 21.2 mm ID x 50 mm length, packed with 10 µm RP C4 Butyl Wide Pore particles featuring a 300 Å pore size. The large internal diameter is designed for high sample loading and fraction collection, while the shorter 50 mm length is ideal for rapid purification cycles, preparative method development, and process screening. Typical flow rates are approximately 15–40 mL/min depending on system capabilities, mobile phase conditions, and sample characteristics.
This column is specifically intended for preparative purification of proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules. The combination of wide pore architecture, fully endcapped C4 chemistry, and preparative dimensions provides high loading capacity, excellent analyte recovery, and reproducible purification performance. The shorter column format is particularly advantageous when throughput and purification speed are more important than maximum chromatographic resolution. A specialized preparative HPLC system capable of higher flow rates, larger injection volumes, and fraction collection is required. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
RP C4 Butyl Wide Pore stationary phase
300 Å pore size optimized for biomolecules
Fully double endcapped chemistry
Traditional butyl (C4) bonded phase
Designed for preparative HPLC purification
Excellent recovery of proteins and peptides
High sample loading capacity
10 µm particles for lower operating backpressure
Suitable for higher flow rates
Fast purification cycles with 50 mm column length
Typical Applications
Preparative HPLC purification
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Biopharmaceutical research
Biotechnology research
Process development
Method scouting
Fraction collection
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C4 Butyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 15–40 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for purification rather than routine analytical HPLC
Intended for collection and isolation of purified biomolecules
Optimized for proteins, peptides, enzymes, and other large molecules
Provides substantially higher sample loading capacity than analytical columns
Requires a preparative HPLC system capable of higher flow rates and fraction collection
Wide pore structure improves accessibility and recovery of biomolecules
Short column length supports rapid purification and method development workflows
Special Order Item